Instituto Pedro Nunes
IPN     IPN Incubadora

Fitopatologia: Tests for the detection of Pseudomonas syringae pv. actinidiae

Client

Various Nurseries and Producers

Duration

2 a 4 semanas cada ensaio

Context

During 2018 and 2019 several tests for the detection of Bacterial Canker of Kiwifruit (Pseudomonas syringae pv. actinidiae) were performed in actinidiae samples (kiwifruit) collected from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.

Keywords: Psa, Pseudomonas syringae pv. actinidiae, Bacterial Canker of Kiwifruit, nursery, Phytosanitary Passports

 

Fitopatologia: Tests for the detection of Pine wilt disease

Client

Instituto de Conservação da Natureza e Florestas (ICNF)

Duration

2 a 4 semanas cada ensaio

Context

 During 2018 several tests for the detection of Pine wilt disease (Bursaphelenchus xylophilus) were performed in wood samples collected in the scope of the National Prospection Plan.

Keywords: Pine wilt disease, Bursaphelenchus xylophilus

 

Fitopatologia: Phytosanitary Evaluation of Kiwi Orchard

Client

CONFIDENTIAL

Duration

30 dias

Context

 Field trips and sampling to evaluate the phytosanitary status of the orchard, namely potentially pathogenic fungi in actinide and Pseudomonas syringae pv. actinidiae (Psa). Identification of Diaporthe actinidiae

Keywords: Pseudomonas syringae pv. actinidiae; Psa; Pathogenic Fungi

 

Fitopatologia: Tests for the detection of Fusarium circinatum

Client

Instituto de Conservação da Natureza e Florestas (ICNF) e Fornecedores MFR

Duration

4 semanas cada ensaio

Context

During 2018 and 2019 several tests for the detection of Pitch canker of Pine (Fusarium circinatum) were performed in pine tree samples collected in the scope of the National Prospection Plan.

Keywords: Fusarium circinatum, Fusarium circinatum 

 

Fitopatologia: Tests for the detection of Erwinia amylovora

Client

Various nurseries

Duration

3 a 4 semanas cada ensaio

Context

 During 2018 and 2019 several tests for the detection of Fire Blight (Erwinia amylovora) were performed in pomoidea samples (apple tree, pear tree, quince) collected from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.

Keywords: Erwinia amylovora, Fogo Bacteriano, viveiro, Passaporte Fitossanitário

 

Fitopatologia:Tests for the detection of Citrus tristeza vírus (CTV)

Client

Viveiristas

Duration

2 a 4 semanas cada ensaio

Context

During 2018 and 2019 several hundred testes for the detection of Citrus tristeza vírus (CTV) were performed in citrus samples (orange tree, lemon tree, etc) from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.

Keywords: CTV, Citrus tristeza virus, nursery, Phytosanitary Passports

 

Fitopatologia: Kiwifruit orchard installation: assessment of the phytosanitary status of the soil and plants prior to the installation

Client

Actiglabro, S.A.

Duration

30 dias

Context

In order to install an orchard it is crucial to access the phytosanitary status of the soil since it may contain nematodes and fungi that are harmful to the plants. On the other hand, the acquired plants should be tested for the presence of Pseudomonas syringae pv. actinidiae (Psa), fungi and nematodes prior to their installation.
In accordance, this test was divided in two. Firstly the soil was sampled for nematodes with special attention to those know to be harmful for actinídea. Secondly, the plants were screened for the presence of Pseudomonas syringae pv. actinidiae (Psa), fungi were isolated and identified and nematodes were collected from roots. 

Keywords: Pseudomonas syringae pv. actinidiae (Psa); Nematodes; Fungi; Kiwi.

 

Diversidade de Microorganismos: Analysis of indoor fungal contamination and air quality

Client

Private client

Duration

2 meses

Context

 Analysis and quantification of the fungal load in different inside walls, window frames and in the indoor air inside a bedroom that showed a clear contamination, using different collection methods (air sampling using a microbial air sampler and cotton swab) and different culture media. Identification through macroscopic and microscopic analysis of the fungal pure cultures under optical microscopy, in conjugation with methods of molecular analysis, for example ITS total region amplification by PCR with subsequent sequencing and BLAST.

Keywords: Diversity; Fungi; air quality; fungal indoor contamination

 

Diversidade de Microorganismos: Analysis of microbial diversity by new generation sequencing

Client

Oro Agri Europe, S.A

Duration

60 dias

Context

Characterization of the microbial diversity (Bacteria and Fungi) in soil samples, with identification of the microbial communities present, as well as their relative abundance, associating a prediction about the presence of groups with potentially harmful impact on the environment / ecosystem.

Keywords: Total Microbial Diversity; Bacteria; Fungi; NGS

 

Diversidade de Microorganismos: Analysis of fungi and nematodes diversity by classic methods

Client

Oro Agri Europe, S.A

Duration

60 dias

Context

Analysis of the diversity of cultivable fungi and oomycote through isolation techniques from soil samples using different culture media. Identification through macroscopic and microscopic analysis of cultures under optical microscopy, in conjugation with methods of molecular analysis, for example amplification of the ITS total region by PCR with subsequent sequencing and BLAST.
Analysis of the nematode diversity by total extraction using the tray method. Identification and quantification of total nematodes by observation under the stereoscopic microscope of the resulting suspensions. Identification based on morphological characteristics. Globodera and Heterodera cysts extraction by soil levigate collection and subsequent quantification.

Keywords: Diversity; Fungi; Nematodes; Classical methods 

 

Diversidade de Microorganismos: Analysis of microbial diversity by new generation sequencing

Client

LIPOR: Serviço Intermunicipalizado de Gestão de Resíduos do Grande Porto

Duration

60 dias

Context

 Characterization of the microbial diversity (Bacteria and Fungi) in a substrate sent by LIPOR, with identification of the microbial communities present, as well as their relative abundance, associating a prediction about the presence of groups with potentially harmful impact on the environment / ecosystem.

Keywords: Total Microbial Diversity; Bacteria; Fungi; NGS

 

Analysis of fungal diversity in pine bark substrate

Client

MADECA

Duration

3 meses durante várias estações do ano

Context

 Analysis and quantification of the diversity of cultivable fungi through isolation techniques in pine bark substrate during several steps of the production cycle and in different seasons of the year, using different culture media. Identification through macroscopic and microscopic analysis of the pure cultures under optical microscopy, in conjugation with methods of molecular analysis, for example ITS total region amplification by PCR with subsequent sequencing and BLAST.

Keywords: Diversity; Fungi; substrates


Tests for the detection of Erwinia amylovora

Client

Nurseries

Duration

2017

Context

During 2017 several testes for the detection of Fire Blight (Erwinia amylovora ) were performed in pomoidea samples (apple tree, pear tree, quince) collected from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.

Keywords: Erwinia amylovora, Fire Blight, nursery, Phytosanitary Passports

Tests for the detection of Citrus tristeza vírus (CTV)

Client

Nurseries

Duration

2017

Context

During 2017 several hundred testes for the detection of Citrus tristeza vírus (CTV) were performed in citrus samples (orange tree, lemon tree, etc) from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.

Keywords: CTV, Citrus tristeza virus, nursery, Phytosanitary Passports

Kiwifruit orchard installation: assessment of the phytosanitary status of the soil and plants prior to the installation

Client

Actiglabro, S.A.

Duration

2017

Context

In order to install an orchard it is crucial to access the phytosanitary status of the soil since it may contain nematodes and fungi that are harmful to the plants. On the other hand, the acquired plants should be tested for the presence of Pseudomonas syringae pv. actinidiae (Psa),fungi and nematodes prior to their installation.
/nIn accordance, this test was divided in two. Firstly the soil was sampled for nematodes with special attention to those know to be harmful for actinídea. Secondly, the plants were screened for the presence of Pseudomonas syringae pv. actinidiae (Psa), fungi were isolated and identified and nematodes were collected from roots.

Keywords: Pseudomonas syringae pv. actinidiae (Psa); Nematodes; Fungi; Kiwi.

Tests for the detection of Pseudomonas syringae pv. actinidiae

Client

Nurseries

Duration

2017

Context

During 2017 several testes for the detection of Bacterial Canker of Kiwifruit (Pseudomonas syringae pv. actinidiae) were performed in actinidia samples (kiwi) collected from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.

Keywords: Psa, Pseudomonas syringae pv. actinidiae, Bacterial Canker of Kiwifruit, nursery,
Phytosanitary Passports

Tests for the detection of Pine wilt disease

Client

Instituto de Conservação da Natureza e Florestas (ICNF)

Duration

2017

Context

During 2017 several testes for the detection of Pine wilt disease (Bursaphelenchus xylophilus) were performed in wood samples collected in the scope of the National Prospection Plan.

Keywords: Pine wilt disease, Bursaphelenchus xylophilus

Phytosanitary Evaluation of Kiwi Orchard

Client

CONFIDENCIAL

Duration

2017

Context

Field trips and sampling to evaluate the phytosanitary status of the orchard, namely potentially pathogenic fungi in actinide and Pseudomonas syringae pv. actinidiae (Psa). Typing of Psa isolates and comparison with strains isolated in analyzes carried out in previous years to the same orchard. Issuance of an integrated phytosanitary
assessment report based on analyzes carried out over 3 years.

Keywords: Pseudomonas syringae pv. actinidiae; Psa; Pathogenic Fungi

Tests for the detection of Fusarium circinatum

Client

Instituto de Conservação da Natureza e Florestas (ICNF) e Fornecedores MFR

Duration

2017

Context

During 2017 several testes for the detection of Pitch canker of Pine (Fusarium circinatum) were performed in pine tree samples collected in the scope of the National Prospection Plan.

Keywords: Fusarium circinatum, Fusarium circinatum

Detection of Citrus tristeza virus (CTV)

Client

Nurseries

Duration

2014

Detection of Citrus tristeza virus (CTV) in citrus plants from Portugal according to EPPO standard 7/31, EPPO Bulletin 34, 155-157.

Quality analyses

Client

Amorim Isolamentos, SA

Duration

2014

Analyses of several types of cork agglomerates for screening and quantification of different types of microorganisms.

Detection of Citrus tristeza virus (CTV)

Client

UC Association InProPlant

Duration

2014

Detection of Citrus tristeza virus (CTV) in citrus plants from Portugal according to EPPO standard 7/31, EPPO Bulletin 34, 155-157.

Detection of Citrus tristeza virus (CTV)

Client

Coimbra District Nurseries Association (AVDC)

Duration

2014

Detection of Citrus tristeza virus (CTV) in citrus plants from Portugal according to EPPO standard 7/31, EPPO Bulletin 34, 155-157.

Detection of Fusarium circinatum

Client

Nurseries

Duration

2013-2014

Detection of Fusarium circinatum in conifers from the central region of Portugal according to EPPO standard 7/91 (1), EPPO Bulletin 39, 298-309.

Detection of Fusarium circinatum

Client

Institute of Nature and Forest Conservation, ICNF, I.P.

Duration

2013-2014

Context

National Plan for the Study of Fusarium circinatum

Detection of Fusarium circinatum in conifers from the central region of Portugal according to EPPO standard 7/91 (1), EPPO Bulletin 39, 298-309.

Detection of Globodera

Client

Agrosearch

Duration

2013

Extraction and quantification of nematodes cysts from soil, quantification and determination of the viability of the eggs according to OEPP standard 7/40 (3).

Detection of Bursaphelenchus xylophilus

Client

Santos & Santos, SA

Duration

2013

Detection of Bursaphelenchus xylophilus in pine tree bark and wood shavings according to EPPO standard OEPP PM 7/4(2) and PM7/4(3).