Client
Various Nurseries and Producers
Duration
2 a 4 semanas cada ensaio
Context
During 2018 and 2019 several tests for the detection of Bacterial Canker of Kiwifruit (Pseudomonas syringae pv. actinidiae) were performed in actinidiae samples (kiwifruit) collected from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.
Keywords: Psa, Pseudomonas syringae pv. actinidiae, Bacterial Canker of Kiwifruit, nursery, Phytosanitary Passports
Client
Instituto de Conservação da Natureza e Florestas (ICNF)
Duration
2 a 4 semanas cada ensaio
Context
During 2018 several tests for the detection of Pine wilt disease (Bursaphelenchus xylophilus) were performed in wood samples collected in the scope of the National Prospection Plan.
Keywords: Pine wilt disease, Bursaphelenchus xylophilus
Client
CONFIDENTIAL
Duration
30 dias
Context
Field trips and sampling to evaluate the phytosanitary status of the orchard, namely potentially pathogenic fungi in actinide and Pseudomonas syringae pv. actinidiae (Psa). Identification of Diaporthe actinidiae
Keywords: Pseudomonas syringae pv. actinidiae; Psa; Pathogenic Fungi
Client
Instituto de Conservação da Natureza e Florestas (ICNF) e Fornecedores MFR
Duration
4 semanas cada ensaio
Context
During 2018 and 2019 several tests for the detection of Pitch canker of Pine (Fusarium circinatum) were performed in pine tree samples collected in the scope of the National Prospection Plan.
Keywords: Fusarium circinatum, Fusarium circinatum
Client
Various nurseries
Duration
3 a 4 semanas cada ensaio
Context
During 2018 and 2019 several tests for the detection of Fire Blight (Erwinia amylovora) were performed in pomoidea samples (apple tree, pear tree, quince) collected from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.
Keywords: Erwinia amylovora, Fogo Bacteriano, viveiro, Passaporte Fitossanitário
Client
Viveiristas
Duration
2 a 4 semanas cada ensaio
Context
During 2018 and 2019 several hundred testes for the detection of Citrus tristeza vírus (CTV) were performed in citrus samples (orange tree, lemon tree, etc) from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.
Keywords: CTV, Citrus tristeza virus, nursery, Phytosanitary Passports
Client
Actiglabro, S.A.
Duration
30 dias
Context
In order to install an orchard it is crucial to access the phytosanitary status of the soil since it may contain nematodes and fungi that are harmful to the plants. On the other hand, the acquired plants should be tested for the presence of Pseudomonas syringae pv. actinidiae (Psa), fungi and nematodes prior to their installation.
In accordance, this test was divided in two. Firstly the soil was sampled for nematodes with special attention to those know to be harmful for actinídea. Secondly, the plants were screened for the presence of Pseudomonas syringae pv. actinidiae (Psa), fungi were isolated and identified and nematodes were collected from roots.
Keywords: Pseudomonas syringae pv. actinidiae (Psa); Nematodes; Fungi; Kiwi.
Client
Private client
Duration
2 meses
Context
Analysis and quantification of the fungal load in different inside walls, window frames and in the indoor air inside a bedroom that showed a clear contamination, using different collection methods (air sampling using a microbial air sampler and cotton swab) and different culture media. Identification through macroscopic and microscopic analysis of the fungal pure cultures under optical microscopy, in conjugation with methods of molecular analysis, for example ITS total region amplification by PCR with subsequent sequencing and BLAST.
Keywords: Diversity; Fungi; air quality; fungal indoor contamination
Client
Oro Agri Europe, S.A
Duration
60 dias
Context
Characterization of the microbial diversity (Bacteria and Fungi) in soil samples, with identification of the microbial communities present, as well as their relative abundance, associating a prediction about the presence of groups with potentially harmful impact on the environment / ecosystem.
Keywords: Total Microbial Diversity; Bacteria; Fungi; NGS
Client
Oro Agri Europe, S.A
Duration
60 dias
Context
Analysis of the diversity of cultivable fungi and oomycote through isolation techniques from soil samples using different culture media. Identification through macroscopic and microscopic analysis of cultures under optical microscopy, in conjugation with methods of molecular analysis, for example amplification of the ITS total region by PCR with subsequent sequencing and BLAST.
Analysis of the nematode diversity by total extraction using the tray method. Identification and quantification of total nematodes by observation under the stereoscopic microscope of the resulting suspensions. Identification based on morphological characteristics. Globodera and Heterodera cysts extraction by soil levigate collection and subsequent quantification.
Keywords: Diversity; Fungi; Nematodes; Classical methods
Client
LIPOR: Serviço Intermunicipalizado de Gestão de Resíduos do Grande Porto
Duration
60 dias
Context
Characterization of the microbial diversity (Bacteria and Fungi) in a substrate sent by LIPOR, with identification of the microbial communities present, as well as their relative abundance, associating a prediction about the presence of groups with potentially harmful impact on the environment / ecosystem.
Keywords: Total Microbial Diversity; Bacteria; Fungi; NGS
Client
MADECA
Duration
3 meses durante várias estações do ano
Context
Analysis and quantification of the diversity of cultivable fungi through isolation techniques in pine bark substrate during several steps of the production cycle and in different seasons of the year, using different culture media. Identification through macroscopic and microscopic analysis of the pure cultures under optical microscopy, in conjugation with methods of molecular analysis, for example ITS total region amplification by PCR with subsequent sequencing and BLAST.
Keywords: Diversity; Fungi; substrates
Client
Nurseries
Duration
2017
Context
During 2017 several testes for the detection of Fire Blight (Erwinia amylovora ) were performed in pomoidea samples (apple tree, pear tree, quince) collected from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.
Keywords: Erwinia amylovora, Fire Blight, nursery, Phytosanitary Passports
Client
Nurseries
Duration
2017
Context
During 2017 several hundred testes for the detection of Citrus tristeza vírus (CTV) were performed in citrus samples (orange tree, lemon tree, etc) from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.
Keywords: CTV, Citrus tristeza virus, nursery, Phytosanitary Passports
Client
Actiglabro, S.A.
Duration
2017
Context
In order to install an orchard it is crucial to access the phytosanitary status of the soil since it may contain nematodes and fungi that are harmful to the plants. On the other hand, the acquired plants should be tested for the presence of Pseudomonas syringae pv. actinidiae (Psa),fungi and nematodes prior to their installation.
/nIn accordance, this test was divided in two. Firstly the soil was sampled for nematodes with special attention to those know to be harmful for actinídea. Secondly, the plants were screened for the presence of Pseudomonas syringae pv. actinidiae (Psa), fungi were isolated and identified and nematodes were collected from roots.
Keywords: Pseudomonas syringae pv. actinidiae (Psa); Nematodes; Fungi; Kiwi.
Client
Nurseries
Duration
2017
Context
During 2017 several testes for the detection of Bacterial Canker of Kiwifruit (Pseudomonas syringae pv. actinidiae) were performed in actinidia samples (kiwi) collected from nurseries in the scope of the Prospection Plan from DRAPC to issue phytosanitary passports by DGAV.
Keywords: Psa, Pseudomonas syringae pv. actinidiae, Bacterial Canker of Kiwifruit, nursery,
Phytosanitary Passports
Client
Instituto de Conservação da Natureza e Florestas (ICNF)
Duration
2017
Context
During 2017 several testes for the detection of Pine wilt disease (Bursaphelenchus xylophilus) were performed in wood samples collected in the scope of the National Prospection Plan.
Keywords: Pine wilt disease, Bursaphelenchus xylophilus
Client
CONFIDENCIAL
Duration
2017
Context
Field trips and sampling to evaluate the phytosanitary status of the orchard, namely potentially pathogenic fungi in actinide and Pseudomonas syringae pv. actinidiae (Psa). Typing of Psa isolates and comparison with strains isolated in analyzes carried out in previous years to the same orchard. Issuance of an integrated phytosanitary
assessment report based on analyzes carried out over 3 years.
Keywords: Pseudomonas syringae pv. actinidiae; Psa; Pathogenic Fungi
Client
Instituto de Conservação da Natureza e Florestas (ICNF) e Fornecedores MFR
Duration
2017
Context
During 2017 several testes for the detection of Pitch canker of Pine (Fusarium circinatum) were performed in pine tree samples collected in the scope of the National Prospection Plan.
Keywords: Fusarium circinatum, Fusarium circinatum
Client
Nurseries
Duration
2014
Detection of Citrus tristeza virus (CTV) in citrus plants from Portugal according to EPPO standard 7/31, EPPO Bulletin 34, 155-157.
Client
Amorim Isolamentos, SA
Duration
2014
Analyses of several types of cork agglomerates for screening and quantification of different types of microorganisms.
Client
UC Association InProPlant
Duration
2014
Detection of Citrus tristeza virus (CTV) in citrus plants from Portugal according to EPPO standard 7/31, EPPO Bulletin 34, 155-157.
Client
Coimbra District Nurseries Association (AVDC)
Duration
2014
Detection of Citrus tristeza virus (CTV) in citrus plants from Portugal according to EPPO standard 7/31, EPPO Bulletin 34, 155-157.
Client
Nurseries
Duration
2013-2014
Detection of Fusarium circinatum in conifers from the central region of Portugal according to EPPO standard 7/91 (1), EPPO Bulletin 39, 298-309.
Client
Institute of Nature and Forest Conservation, ICNF, I.P.
Duration
2013-2014
Context
National Plan for the Study of Fusarium circinatum
Detection of Fusarium circinatum in conifers from the central region of Portugal according to EPPO standard 7/91 (1), EPPO Bulletin 39, 298-309.
Client
Agrosearch
Duration
2013
Extraction and quantification of nematodes cysts from soil, quantification and determination of the viability of the eggs according to OEPP standard 7/40 (3).
Client
Santos & Santos, SA
Duration
2013
Detection of Bursaphelenchus xylophilus in pine tree bark and wood shavings according to EPPO standard OEPP PM 7/4(2) and PM7/4(3).